Thawing of samples
In order to obtain increased motility, it is essential to follow the thawing protocols recommended by Institut Marquès:
- After having removed the rod on the transport tank and checked the data with the report issued by Institut Marques, place the vial immediately in a water bath at 37 ° C for 5 minutes.
- In the case of cryotubes, slightly loosen the cap to release pressure.
- If the case of standard sperm samples, process the sample using the protocol established in each centre (density gradients, wash and spin, etc.)
- In the case of IUI-ready samples, move the sample thawed to a sterile tube and keep at room temperature until insemination (insemination is recommended as soon as possible after thawing the sample). For In Vitro Fertilisation cycles, Institut Marquès recommends washing with growth medium before ICSI or eggs insemination.