Other micromanipulation techniques
The micromanipulation techniques are complementary techniques to In Vitro Fertilisation for special cases to to improve results.
Assisted hatching or ‘zona drilling’ consist in perfoming a small hole in the zona pellucida of the embryo (a layer covering the embryo). As women get older, the zona pellucida may become hardened and tougher making it difficult for the embryos to hatch. With assisted hatching, once the embryo reaches the blastocyst stage, it can break out of it’s zona pellucidae much easier thus facilitaing better implantation rates.
How is the assisted hatching performed?
For the assisted hatching is necessary to hold the embryo with a holding pipette (a thin glass tube) and an small hole in the zona pellucidae is performed using acidified solution (acid tyrodes solution).
Microsurgical fragment removal
The microsurgical fragment removal procedure is one of the newest assisted reproduction techniques designed to eliminate cellular fragments that could adversely affect embryo development. A hole is made in the zona pellucidae and most of the fragments are aspirated and eliminated.
What exactly are the cellular fragments?
Human embryos may have celullar fragments in between the blastomeres which fragments of cytoplasm sourrounded by a membrane that have been lost during embryo division. These fragments are originated during the embryo cleavage process and interfere considerably with their further development.
Why is considered microsurgery?
Because it is performed with the microscope under high magnification and it can change embryo’s appearance and return them to their original pregnancy potencial.
The polarizer is an optical system integrated in a microscope based on the different polarization indexes of light. With this technique we can observe the most important structures in the oocyte, such as the meiotic spindle” (inner structure of the egg where its genetic material is concentrated) to prevent from damaging it in the microinjection process, thus increasing the fertilisation rate.
We can also identify the stages of ovocitary maturity perfectly, analyze the oocyte very precisely after the freezing and unfreezing procedures and improve the efficiency of the polar body and embryo biopsy techniques in cases of Preimplantational Genetic Diagnose (PGD).